RNA Sequencing

RNA sequencing of murine mammary epithelial stem-like cells (HC11) undergoing lactogenic differentiation and its comparison with embryonic stem cells

16:35 11 April in RNA Sequencing
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Understanding of transcriptional networks specifying HC11 murine mammary epithelial stem cell-like cells (MEC) in comparison with embryonic stem cells (ESCs) and their rewiring, under the influence of glucocorticoids (GC) and prolactin (PRL) hormones, is critical for elucidating the mechanism of lactogenesis. In this data note,...

Plant pathogen interaction study on rice published in Nature Scientific Reports

12:01 06 February in RNA Sequencing
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Alterations in rice chloroplast integrity, photosynthesis and metabolome associated with pathogenesis of Rhizoctonia solani Sheath blight disease is caused by a necrotrophic fungal pathogen Rhizoctonia solani and it continues to be a challenge for sustainable rice cultivation. In this study, we adopted a multi-pronged approach to understand the intricacies...

De novo sequencing and characterization of defense transcriptome responsive to Pythium aphanidermatum infection in Curcuma longa L.

13:07 29 March in RNA Sequencing, Uncategorized
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  Defence transcriptomes responsive to Pythium aphanidermatum were analyzed in Curcuma longa. 2978 transcripts were differentially expressed in response to P. aphanidermatum infection. DEGs encoding NBS-LRRs, receptor kinases and WRKYs were highly enriched during pathogen attack. ET and JA signaling constitute a synergistic defence response...

Sequencing, de novo assembly, functional annotation and analysis of Phyllanthus amarus leaf transcriptome using the Illumina platform is published in Frontiers in Plant Sciences.

11:31 28 January in RNA Sequencing, Uncategorized
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P. amarus leaf transcriptome was sequenced using the Illumina Miseq platform. 85,927 non-redundant “uni-transcript” sequences with an average length of 1548 bp, from 18,060,997 raw reads were assembled. Sequence similarity analyses and annotation of these uni-transcripts were performed against databases like green plants non-redundant (nr)...