RNA Sequencing

Plant pathogen interaction study on rice published in Nature Scientific Reports

12:01 06 February in RNA Sequencing
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Alterations in rice chloroplast integrity, photosynthesis and metabolome associated with pathogenesis of Rhizoctonia solani Sheath blight disease is caused by a necrotrophic fungal pathogen Rhizoctonia solani and it continues to be a challenge for sustainable rice cultivation. In this study, we adopted a multi-pronged approach to understand the intricacies...

De novo sequencing and characterization of defense transcriptome responsive to Pythium aphanidermatum infection in Curcuma longa L.

13:07 29 March in RNA Sequencing, Uncategorized
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  Defence transcriptomes responsive to Pythium aphanidermatum were analyzed in Curcuma longa. 2978 transcripts were differentially expressed in response to P. aphanidermatum infection. DEGs encoding NBS-LRRs, receptor kinases and WRKYs were highly enriched during pathogen attack. ET and JA signaling constitute a synergistic defence response...

Sequencing, de novo assembly, functional annotation and analysis of Phyllanthus amarus leaf transcriptome using the Illumina platform is published in Frontiers in Plant Sciences.

11:31 28 January in RNA Sequencing, Uncategorized
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P. amarus leaf transcriptome was sequenced using the Illumina Miseq platform. 85,927 non-redundant “uni-transcript” sequences with an average length of 1548 bp, from 18,060,997 raw reads were assembled. Sequence similarity analyses and annotation of these uni-transcripts were performed against databases like green plants non-redundant (nr)...