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Plant pathogen interaction study on rice published in Nature Scientific Reports

12:01 06 February in RNA Sequencing

Alterations in rice chloroplast integrity, photosynthesis and metabolome associated with pathogenesis of Rhizoctonia solani Sheath blight disease is caused by a necrotrophic fungal pathogen Rhizoctonia solani and it continues to be a challenge for sustainable rice cultivation. In this study, we adopted a multi-pronged approach to understand the intricacies...

De novo sequencing and characterization of defense transcriptome responsive to Pythium aphanidermatum infection in Curcuma longa L.

13:07 29 March in RNA Sequencing, Uncategorized

  Defence transcriptomes responsive to Pythium aphanidermatum were analyzed in Curcuma longa. 2978 transcripts were differentially expressed in response to P. aphanidermatum infection. DEGs encoding NBS-LRRs, receptor kinases and WRKYs were highly enriched during pathogen attack. ET and JA signaling constitute a synergistic defence response...

Sequencing, de novo assembly, functional annotation and analysis of Phyllanthus amarus leaf transcriptome using the Illumina platform is published in Frontiers in Plant Sciences.

11:31 28 January in RNA Sequencing, Uncategorized

P. amarus leaf transcriptome was sequenced using the Illumina Miseq platform. 85,927 non-redundant “uni-transcript” sequences with an average length of 1548 bp, from 18,060,997 raw reads were assembled. Sequence similarity analyses and annotation of these uni-transcripts were performed against databases like green plants non-redundant (nr)...

Transcriptome analysis of Solanum melongena L. (eggplant) fruit to identify putative allergens and their epitopes

14:09 15 January in de novo transcriptome analysis

Complete transcriptome catalogue of brinjal fruit was generated using NGS technology. Brinjal transcripts showed significant similarity with other plant proteins. Putative allergen sequences of eggplant matched with 48 different types of existing allergens. B-cell epitopes were identified using BepiPred linear epitope prediction tool.  ...