Sequencing, de novo assembly, functional annotation and analysis of Phyllanthus amarus leaf transcriptome using the Illumina platform is published in Frontiers in Plant Sciences.
P. amarus leaf transcriptome was sequenced using the Illumina Miseq platform. 85,927 non-redundant “uni-transcript” sequences with an average length of 1548 bp, from 18,060,997 raw reads were assembled. Sequence similarity analyses and annotation of these uni-transcripts were performed against databases like green plants non-redundant (nr) protein database, Gene Ontology (GO), Clusters of Orthologous Groups (COG), PlnTFDB, KEGG databases. As a result, 69,394 GO terms, 583 enzyme codes, 134 KEGG maps and 59 Transcription Factor families were generated. In addition, 65,273 simple sequence repeats (SSRs) were also identified. Our study provides the largest genetic resource that will lead to drug development and pave the way in deciphering various secondary metabolite biosynthetic pathways in P. amarus, especially those conferring the medicinal attributes of this potent herb.