Hybrid Genome Assembly Microbial Genome

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A fungal genome of 40Mb was recently sequenced and assembled by our team. We used HiSeq 2500 for sequencing of PE and MP libraries. We also used 4 SMRT cells data of PacBIo RS II. Both the data sets were used to assemble the fungal genome. We identified missing regions which were not reported earlier. We were also able to discover genome repeats and variants in this Indian isolate of fungus.