Nucleome Informatics is the only company in India to offer sequencing services on PacBio Sequel II sequencer. We offer Whole-genome sequencing service of Microbe, Plant and Animal Genomes.
HMW gDNA and Library QC
HMW gDNA Shearing and Size selection
Library Insert Size
Sequencing on Sequel II platform
We use various tools to assemble the genomes. We build contig level assembly with PacBio Sequel II data and scaffold the genome with Optical map and HiC data after multiple rounds of polishing, gap filling and curation. Assessment of assembly is done using assembly stats like Contig N50, Scaffold N50, No of Gaps, Base accuracy, BUSCO Score, RNA Seq mappability, Phase Block resolution and Chromosomal level continuity. We offer the following type of assembly and scaffolding services;
For any query and more information on our services, please contact our sales team at salessupport @ nucleomeinfo.com.
Nucleome provides full-length transcriptome sequencing i.e ISO Sequencing on Pacbio Sequel II platform. Nucleome Informatics is the only service provider of ISO Sequencing on Sequel II in India. The PacBio Iso-Seq method is an end-to-end workflow for sequencing and analyzing full-length transcript isoforms. Our Iso-Seq analysis enables you to detect novel transcripts and genes, identify fusion genes, annotate isoforms and alternative splicing events.
Generate full-length transcript sequences up to ~15kb
ISO Seq Service WorkFLow:
1. Convert RNA into cDNA
2. cDNA SMRTbell library preparation
3. Sequence on the Sequel II System
4. Generate circular consensus sequences (CCS)
5. Discover isoforms de novo with Iso-Seq analysis
ISO Seq Applications:
-Typically whole-transcriptome, non- quantitative
-Often included in de novo genome assembly projects
-Single tissue to several tissues
-Generates reference transcriptome for downstream RNA-seq studies
Gene-level Isoform Discovery
-Typically targeted, either cDNA amplicons or target capture
-Useful for detecting gene fusions, SNVs, allele-specific expression
-Cost-effectively multiplex many samples per single SMRT Cell
-Relative quantitation possible
1 ug of high qulaity total RNA, RIN >7 and Concentration should be more than 25ng per ul
– Has not been exposed to high temperatures (e.g.: > 65°C for 1 hour can cause a detectable decrease in sequence quality) or pH extremes (< 6 or > 9).
– Has an OD260/OD280 ratio between 2.0 and 2.2. – Has an OD260/OD230 ratio between 1.8 and 2.1. – Has a RIN number ≥ 8 (Recommended).
– Has not been exposed to intercalating fluorescent dyes or ultraviolet radiation. SYBR dyes are not RNA damaging, but do avoid ethidium bromide.
– Does not contain denaturants (e.g., guanidinium salts or phenol) or detergents (e.g., SDS or Triton‐X100).
– Does not contain carryover contamination from the original organism/tissue (e.g., heme, humic acid, polyphenols, etc.).
– Note: RNA samples should only be shipped with dry ice.
Contact our team now to receive the quote and place the order.
Nucleome is the only service provider in South Asia that offers Single Molecule, Real-Time (SMRT) Sequencing on PacBio Sequel II that generates the long, accurate, single-molecule reads you need to comprehensively characterize samples with complex variation. With SMRT Sequencing you can:
Highly accurate long reads – HiFi reads – with the single-molecule resolution are ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly so that you can:
Full-length Metagenome assembly and functional profiling on PacBio Sequel II
HiFi sequencing on the PacBio Sequel II System enables complete microbial community profiling of complex metagenomic samples using whole genome shotgun sequences. With HiFi sequencing, highly accurate long reads overcome the challenges posed by the presence of intergenic and extragenic repeat elements in microbial genomes, thus greatly improving phylogenetic profiling and sequence assembly.
HiFi sequencing can be used even for sample types with limited starting material, allowing for reference quality MAGs, discovery of novel genes without the need for de novo assembly, identification of biochemical pathways, and resolution of taxa below the species level with confidence.
Output data in standard file formats, (BAM and FASTA/Q) for seamless integration with downstream analysis tools – Perform taxonomic classification and functional gene profiling using QIIME and MEGAN
– Perform gene prediction and discovery using FragGeneScan and Prodigal
Learn more about workflows for these types of complex populations
– Perform metagenomic shotgun assembly directly with HiFi reads using HiCanu
Full length 16s Amplicon Sequencing by Nucleome
Nucleome is the only service provider in South Asia that offers Single Molecule, Real-Time (SMRT) Sequencing on PacBio Sequel II that generates full length 16s amplicon sequencing. The ~1500 bp 16S rRNA gene comprises nine variable regions interspersed throughout the highly conserved 16S sequence. Sequencing the partial gene was originally accomplished by Sanger sequencing. This required cloning genes, generating, and assembling two to three reads per clone, and producing limited sampling depth at high cost and effort. The partial 16s rRNA gene sequencing misses to sequence the entire taxa of the bacterial community.
DIFFERENT SUB-REGIONS SHOWED BIAS IN THE BACTERIAL TAXA THEY WERE ABLE TO IDENTIFY
Full V1–V9 region: the only way to resolve ALL the clades that may be present in a complex population. Order full length 16s Amplicon sequencing to Nucleome and discover Strain-level identification of bacterial communities with the unprecedented accuracy of PacBio HiFi reads.