Wildlife Genomics

Home / Services / Wildlife Genomics

Wildlife Genomics

 

Globally wildlife species are facing anthropogenic pressures directly or indirectly such as habitat loss and alteration,direct mortality due to exploitation, introduction of invasive species,pollution and climate change. Under these conditions, it is crucial to improve our basic understanding of wildlife species and populations, including evolutionary history, adaptation to changing local environments and ecological interactions which further can be useful in conservation and management efforts in conserving the species. Advancement in molecular technologies like genome sequencing and/or SNP genotyping has revolutionized the field of wild life conservation, allowing high throughput sequencing to be applied in nearly any organism including rare and elusive species.

Applications:

  1. Identify populations and units for conservation
  2. Assess inbreeding and genomic diversity
  3. Understand the demographic histories, population structure and connectivity
  4. Estimate effective populations sizes
  5. Detect hybridization and admixture patterns
  6. Identify the potentiality of populations to persist and adapt to changes in the environment

 

Services provided at Nucleome:

  1. Denovo and Reference genome sequencing and chromosomal level assembly generation using combination of
  • Long reads data using PacBio Sequel II for contig level assembly
  • Optical mapping using Bionano Saphyr for scaffolding
  • Hi-C using Illumina NovaSeq 6000 for super scaffolding
  • Short read paired end data using Illumina NovaSeq 6000 for gap filling and genome survey

2.Genome resequencing for discovery of SNP’s using NovaSeq 6000 and structural variants using PacBio Sequel II

3.Transcriptome sequencing including:

  • Full length transcriptome and Isoforms using PacBio Sequel II
  • Targeted RNA sequencing (lncRNA, mRNA, non-coding, and miRNA) using NovaSeq 6000
  1. SNP genotyping, Genotyping-by-Sequencing, microarrays (Catalogue arrays, Custom chip development and Custom Axiom and Illumina arrays for canine, bovine, equine, feline, ovine, and porcine) using NovaSeq 6000
  2. Metagenomics including:
  • Shotgun Metagenome, 16s rRNA Amplicon (V3-V4), 18s rRNA, and ITS using Illumina NovaSeq 6000
  • Full length Shotgun Metagen ome and 16s full length (V1-V9) sequencing using PacBio Sequel II

Partnered with Vertebrate Genome
Project of the G10k Consortium

 

 

@Yadvendradev Jhala

@Devesh Gadhvi

 

@Mihir Goodbole