circularRNA Sequencing

Nucleome Informatics is a leading service provider of circular RNA Sequencing in India. The circRNA digitalization analysis based on HiSeq high-throughput sequencing uses the SBS-sequencing by synthesis, which can decrease the loss of nucleotides caused by the secondary structure. It is also powerful for its small requirement of sample quantity, high throughput, high accuracy with an easily operated automatic platform.

Sample Requirement

  • Total RNA amount: ≥ 2.0 μg; RNA concentration: ≥ 50 ng/μl (For human and mouse, 200 ng could be accepted with risk.)
  • RIN value ≥ 6.3 for plants and fungi; RIN value ≥ 6.8 for animals
  • OD260/280 ≥ 2.0, OD260/230 ≥ 2.0, without degradation and DNA contamination

Recommended Sequencing Depth

  • ≥ 20 Million reads per sample of 2×150 bp on HiSeq 2500

Sequencing Workflow

The experimental procedure of circRNA sequencing is shown below:

Deliverables

Option 1: Without Bioinformatics Analysis
  • Sample QC Report
  • Raw Data in .fastQ Format
  • Raw Data Statistics
  • Clean Data QC
  • Sequencing and Raw Data QC Report
 
Option 2: With Bioinformatics Analysis
  • Sample QC Report
  • Raw Data in .fastQ Format
  • Raw Data Statistics
  • Clean Data QC
  • Sequencing and Raw Data QC Report
  • Alignment of Clean Reads Over Reference Genome
  • circRNA Identification, Quantification and DGE
  • circRNA Source Gene Analysis
  • GO Enrichment Analysis
  • KEGG Enrichment Analysis
  • miRNA Binding Site Analysis
  • Tissue Specific Analysis

Turnaround Time (TAT)

3 to 4 months from the date samples pass QC.