PacBio calls their technology SMRT sequencing – single molecule, real-time. Unlike most other sequencing technologies, it doesn’t require clonal amplification of DNA – it sequences single molecules. The real-time nature of PacBio leads to three distinct advantages. First, the reads are quite fast, with runs lasting from 30 minutes to three hours (rather than days). Second, the reads are substantially longer than most other commercially available sequencing platforms (including Sanger-based sequencers), with a mean of ~15 kb. Third, the movie captures information about the rate of nucleotide incorporation, which can be used to determine the modification status of the template nucleotide (e.g. 5-mC, 5-hmC, etc.). The raw read error rate is substantially higher at around 14% compared with the 0.1 to 1% error rate of other leading systems. However, unlike the others, the error model is stochastic, so very high quality reads across all bases can be achieved in the consensus sequence. Additionally, the SMRT sequencing chemistry can sequence regions of high GC content, leading to much more uniform coverage of the genome. Given this unique mix of strengths and weaknesses, Nucleome use PacBio RS II latest chemistry for sequencing of small genomes (e.g., viral and bacterial), difficult to sequence regions or genomes (e.g., high GC content), stand alone denovo genome sequencing of larger genomes, hybrid assembly, gap filling, scaffolding, native detection of modified bases or any application where ultra long reads are necessary (e.g., full-length cDNAs).