Sequel II System

The Sequel II is the aptly named smarter, high quality producing follow-on to the Sequel system. The sequel II can generate upto 100Gb CLR data per SMRT cell. We use this instrument for de novo genome sequencing of larger genomes where the scientists want to generate higher coverage of 60X or more. The Sequel II system also delivers HiFi longer read length of higher quality for de novo genome sequencing, low input DNA protocol, SV discovery, Variant discovery, ISO Sequencing, 16s Full length RNA Sequencing, shotgun metagenome sequencing and targeted Amplicon sequencing applications.

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Illumina NovaSeq 6000

Illumina NovaSeq 6000 Sequencing System installed at Nucleome’s labs NKC Centre for genomics research Hyderabad and CSIR-IHBT, Palampur are the world’s largest short read genome sequencing instrument designed to do it all. Use it how you want to. Now Nucleome can offer every genome, application, project and throughput are at your fingertips for impactful studies to change the future of genomics research.


GeneTitan® Multi-Channel (MC) Instrument for expression and genotyping can be used for hybridization, washing, and imaging in a single instrument. GeneTitan® Instruments together with Affymetrix® High-Throughput (HT) Array Plates provide the hands-free, automated solution for microarray processing. With a broad selection of array plate formats, we use this instrument from discoveries through genome-wide SNP genotyping, to comprehensive explorations of gene expression profiles relating to important biological phenotypes, such as disease or drug response. We frequently use this instrument for large scale custom SNP genotyping programs using Axiom myDesign genotyping array solutions.

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Bionano Saphyr System

The Nucleome Informatics is the first Indian company to offer optical mapping on the Bionano Genomics Saphyr system. The Saphyr system uses a non-sequencing based optical mapping technology to analyse ultra-long strands of genomic DNA. This DNA is fluorescently labelled with the new Direct Labelling (DLS) technology and is imaged after individual DNA molecules are separated in thousands of channels on a nano-fluidic chip.
Large structural variants, including insertions and deletions, translocations and inversions at a genomic scale, can be analysed with this system. A favourable method for researchers interested in, for example, plant trait development, domestication, or polyploidy. With high-quality software and improved computational resources, assembly, scaffolding, variant calling, and annotation is readily available for our customers.
The Saphyr helps construct high precision genome maps, which can be used to build de novo genome assembly and superscaffolding of contig level genomes. The power of this combination can be found in an increase in the contiguity of the assembly to benefit both practically and economically.

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HiSeq 2500

The HiSeq 2500 (1T upgrade), an upgrade of the original HiSeq 2000, has two separate operating modes, both using the standard SBS chemistry. The ‘high throughput mode’ can generate up to 1Tb per 6 days run with SBS v4 chemistry. For those willing to pay a premium for faster results, there is also a ‘rapid mode’ that is capable of generating 180 Gb in 40 hours. The new V4 reagents increase the number of clusters by 33% compared to the TruSeq SBS Kit V3, adding additional capacity for counting assays. It uses modified single-channel flow cells, onboard amplification and ‘fast’ chemistry to achieve the faster run times. We use this instrument for almost every NGS application starting from Genome, Exome, transcriptome, epigenome and Metagenome sequencing as this instrument and chemistry generates highest data quality and output.

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The MiSeq, which launched in the fall of 2011, is Illumina’s desktop or “personal sequencing system”. It is a smaller, faster and lower output sequencer, aimed primarily at the emerging sequencing-based diagnostics market. It is a self-contained system with onboard cluster generation, simplified reagent handling, and tight integration with Base Space, Illumina’s cloud-based data analysis platform. In November 2013 Illumina launched an FDA-cleared version of the instrument, called the MiSeqDx. Its MiSeq SBSv3 kit produces 2×300 bp read length and up to 15 Gb data in one run. We use this technology for denovo genome sequencing of smaller genomes, 16s Amplicon sequencing and sequencing of cancer panels.

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Illumina iScan

The iScan system utilises Bead-Array microarray technology for a broad range of DNA and RNA analysis applications. Each bead on Illumina arrays is covered with hundreds of thousands of copies of specific oligos ​ that act as the capture sequences in one of our array-based assays.​Bead-Array product density advancements have increased genomic coverage for whole-genome genotyping, resolution for cytogenetics and CNV detection, and sample throughput for gene expression, DNA methylation, and focused genotyping products.

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