de novo transcriptome analysis Tag

Sequencing, de novo assembly, functional annotation and analysis of Phyllanthus amarus leaf transcriptome using the Illumina platform is published in Frontiers in Plant Sciences.

11:31 28 January in RNA Sequencing, Uncategorized

P. amarus leaf transcriptome was sequenced using the Illumina Miseq platform. 85,927 non-redundant “uni-transcript” sequences with an average length of 1548 bp, from 18,060,997 raw reads were assembled. Sequence similarity analyses and annotation of these uni-transcripts were performed against databases like green plants non-redundant (nr)...

Transcriptome analysis of Solanum melongena L. (eggplant) fruit to identify putative allergens and their epitopes

14:09 15 January in de novo transcriptome analysis

Complete transcriptome catalogue of brinjal fruit was generated using NGS technology. Brinjal transcripts showed significant similarity with other plant proteins. Putative allergen sequences of eggplant matched with 48 different types of existing allergens. B-cell epitopes were identified using BepiPred linear epitope prediction tool.  ...