PacBio Revio System

Sequel II System

The Sequel II is the aptly named smarter, high quality producing follow-on to the Sequel system. The sequel II can generate upto 100Gb CLR data per SMRT cell. We use this instrument for de novo genome sequencing of larger genomes where the scientists want to generate higher coverage of 60X or more. The Sequel II system also delivers HiFi longer read length of higher quality for de novo genome sequencing, low input DNA protocol, SV discovery, Variant discovery, ISO Sequencing, 16s Full length RNA Sequencing, shotgun metagenome sequencing and targeted Amplicon sequencing applications.

Illumina NovaSeq 6000

Illumina NovaSeq 6000 Sequencing System installed at Nucleome’s labs NKC Centre for genomics research Hyderabad and CSIR-IHBT, Palampur are the world’s largest short read genome sequencing instrument designed to do it all. Use it how you want to. Now Nucleome can offer every genome, application, project and throughput are at your fingertips for impactful studies to change the future of genomics research.

GeneTitan

GeneTitan® Multi-Channel (MC) Instrument for expression and genotyping can be used for hybridization, washing, and imaging in a single instrument. GeneTitan® Instruments together with Affymetrix® High-Throughput (HT) Array Plates provide the hands-free, automated solution for microarray processing. With a broad selection of array plate formats, we use this instrument from discoveries through genome-wide SNP genotyping, to comprehensive explorations of gene expression profiles relating to important biological phenotypes, such as disease or drug response. We frequently use this instrument for large scale custom SNP genotyping programs using Axiom myDesign genotyping array solutions.

Bionano Saphyr System

The Nucleome Informatics is the first Indian company to offer optical mapping on the Bionano Genomics Saphyr system. The Saphyr system uses a non-sequencing based optical mapping technology to analyse ultra-long strands of genomic DNA. This DNA is fluorescently labelled with the new Direct Labelling (DLS) technology and is imaged after individual DNA molecules are separated in thousands of channels on a nano-fluidic chip.
Large structural variants, including insertions and deletions, translocations and inversions at a genomic scale, can be analysed with this system. A favourable method for researchers interested in, for example, plant trait development, domestication, or polyploidy. With high-quality software and improved computational resources, assembly, scaffolding, variant calling, and annotation is readily available for our customers.
The Saphyr helps construct high precision genome maps, which can be used to build de novo genome assembly and superscaffolding of contig level genomes. The power of this combination can be found in an increase in the contiguity of the assembly to benefit both practically and economically.

The Ion GeneStudio S5 Plus System

Ion GeneStudio S5 Plus System: Flexible, Scalable, and Efficient Sequencing for Diverse Applications

The Ion GeneStudio S5 Plus System is a semiconductor-based next-generation sequencing (NGS) platform, offering simplified and efficient targeted sequencing workflows. Designed for both small and large projects, it supports a wide range of applications, making it an ideal solution for service providers catering to diverse research and clinical needs.

With cartridge-based plug-and-play reagents and a selection of Ion 5 Series chips (Ion 510–550), this system enables high-throughput sequencing with flexibility and ease of use. It’s perfect for delivering rapid results with minimal setup time, enabling you to focus on advancing your research.

Key Features:
– Flexible Chip Selection: Achieve 2–130 million reads per run by selecting the chip that suits your application and throughput requirements.
– Simplified Workflow: Cartridge-based reagents streamline setup, reducing complexity and saving time.
– Fast Turnaround: Complete sequencing runs and data analysis within hours.

Applications for Service Providers:
The Ion GeneStudio S5 Plus System supports a wide array of research and clinical applications, including:

– Targeted Gene Panels: Comprehensive sequencing for disease-specific panels such as oncology, cardiology, and rare genetic disorders.

– AgriSeq Panels: Various low-density SNP panels are available for agrigenomics research and molecular breeding
– Exome Sequencing: High-coverage sequencing is used to detect coding region variants.
– RNA-Seq & Transcriptomics: Analyze gene expression profiles for biomarker discovery and functional genomics.
– Microbial Genomics: Whole-genome sequencing of microbial strains for epidemiology and pathogen identification.
– Metagenomics: Study microbial diversity and dynamics in complex samples.
– Inherited Disease Studies: Identify genetic variants associated with inherited conditions.
– Oncology Applications: Detect somatic and germline mutations for translational cancer research.

Contact the Nucleome team if you wish to use this powerful genomic solution in your research.

MiSeq

The MiSeq, which launched in the fall of 2011, is Illumina’s desktop or “personal sequencing system”. It is a smaller, faster and lower output sequencer, aimed primarily at the emerging sequencing-based diagnostics market. It is a self-contained system with onboard cluster generation, simplified reagent handling, and tight integration with Base Space, Illumina’s cloud-based data analysis platform. In November 2013 Illumina launched an FDA-cleared version of the instrument, called the MiSeqDx. Its MiSeq SBSv3 kit produces 2×300 bp read length and up to 15 Gb data in one run. We use this technology for denovo genome sequencing of smaller genomes, 16s Amplicon sequencing and sequencing of cancer panels.

Illumina iScan

The iScan system utilises Bead-Array microarray technology for a broad range of DNA and RNA analysis applications. Each bead on Illumina arrays is covered with hundreds of thousands of copies of specific oligos ​ that act as the capture sequences in one of our array-based assays.​Bead-Array product density advancements have increased genomic coverage for whole-genome genotyping, resolution for cytogenetics and CNV detection, and sample throughput for gene expression, DNA methylation, and focused genotyping products.