T2T Genome Assembly of Goldlined Seabream

Telomere to Telomere genome assembly of Goldlined Seabream is published.

We are excited to share a significant milestone in Indian aquaculture genomics: the chromosome-level genome sequencing and assembly of Rhabdosargus sarba (goldlined seabream), a highly adaptable, euryhaline marine fish with outstanding potential for aquaculture.

This project was led by scientists at ICAR-CIBA and powered by the technical expertise of Nucleome, who served as the genomics service provider. Heartfelt thanks to our client, ICAR-CIBA, and all team members for their outstanding support and collaboration.

Key outcomes from the project:

• Genome sequencing and assembly of R. sarba was performed using a combination of PacBio HiFi (long-read), Illumina (short-read), and Hi-C (chromatin interaction) sequencing technologies.

• The final assembled genome spans 764.59 Mb in 31 scaffolds, with an N50 length of 33.98 Mb.

• Repeat elements constitute 28.71% of the genome.

• Gene prediction based on ab initio methods and transcriptome data identified 26,913 protein-coding genes.

• Functional annotation revealed participation of these genes in 332 pathways, covering vital biological processes.

• This assembly provides an excellent foundation for further exploration of genetic resources, molecular breeding, and adaptive evolution in R. sarba—a species already recognized for its strong adaptability and aquaculture value.

This high-quality reference genome will drive future research in genetic improvement, conservation, and sustainable aquaculture production for R. sarba and related species.

Thank you again to ICAR-CIBA and every member of the project team for making this achievement possible.