RNA-Seq of Rice Yellow Stem Borer, published in G3 : Genes, Genomes and Genetics.
RNA-Seq of Rice Yellow Stem Borer, Scirpophaga incertulas Reveals Molecular Insights During Four Larval Developmental Stages.
The yellow stem borer (YSB), Scirpophaga incertulas is a prominent pest in the rice cultivation causing serious yield losses. To understand and identify the genes involved in YSB development and infestation, so as to design pest control strategies, we performed de novo transcriptome at 1st, 3rd, 5th and 7th larval developmental stages employing Illumina Hi-seq. Identification of stage specific transcripts made possible to determine the essential processes of larval development. Comparative transcriptome analysis revealed that YSB has not much evolved in detoxification mechanism but showed presence of distinct RNAi machinery. This is the first report on characterization of YSB transcriptome and identification of genes involved in key processes which will help researchers and industry to devise novel pest control strategies. This study opens a new avenue to develop next generation resistant rice using RNAi or genome editing approaches.