De Novo Transcriptome Analysis of Allium cepa L. (Onion) published in PLOS ONE
From the 99,074,309 high-quality raw reads that were generated from onion bulb, 49 different functional groups of genes were identified. With BLASTx top hit analysis, a total of 2,511 homologous allergenic sequences were found, which had 37 to 100% similarity with 46 different types of allergens existing in the database. From the 46allergens, more than 500 B-cell linear epitopes were identified. This is the first comprehensive insight into the transcriptome of onion bulb tissue using the NGS technology, which can be used to map IgE epitopes and prediction of structures and functions of various proteins.